A recombinant multimeric immunoglobulin expressed in rice shows assembly-dependent subcellular localization in endosperm cells.

@article{Nicholson2005ARM,
  title={A recombinant multimeric immunoglobulin expressed in rice shows assembly-dependent subcellular localization in endosperm cells.},
  author={Liz Nicholson and Pablo Gonzalez-Melendi and Craig J van Dolleweerd and Heidi Tuck and Yolande Perrin and Julian K-C Ma and Rainer Fischer and Paul Christou and Eva Stoger},
  journal={Plant biotechnology journal},
  year={2005},
  volume={3 1},
  pages={115-27}
}
To investigate the role of subunit assembly in the intracellular deposition of multimeric recombinant proteins, we expressed a partially humanized secretory immunoglobulin in rice endosperm cells and determined the subcellular locations of the assembled protein and its individual components. Transgenic rice plants expressing either individual subunits or all the subunits of the antibody were generated by particle bombardment, and protein localization was determined by immunoelectron microscopy… CONTINUE READING