A receptor-like cytoplasmic kinase targeted by a plant pathogen effector is directly phosphorylated by the chitin receptor and mediates rice immunity.

Abstract

CERK1 is a lysine motif-containing plant pattern recognition receptor for chitin and peptidoglycan. Chitin recognition by OsCERK1 triggers rapid engagement of a rice MAP kinase cascade, which leads to defense response activation. How the MAP kinase cascades are engaged downstream of OsCERK1 remains obscure. Searching for host proteins that interact with Xoo1488, an effector of the rice pathogen Xanthomonas oryzae, we identified the rice receptor-like cytoplasmic kinase, OsRLCK185. Silencing OsRLCK185 suppressed peptidoglycan- and chitin-induced immune responses, including MAP kinase activation and defense-gene expression. In response to chitin, OsRLCK185 associates with, and is directly phosphorylated by, OsCERK1 at the plasma membrane. Xoo1488 inhibits peptidoglycan- and chitin-induced immunity and pathogen resistance. Additionally, OsCERK1-mediated phosphorylation of OsRLCK185 is suppressed by Xoo1488, resulting in the inhibition of chitin-induced MAP kinase activation. These data support a role for OsRLCK185 as an essential immediate downstream signaling partner of OsCERK1 in mediating chitin- and peptidoglycan-induced plant immunity.

DOI: 10.1016/j.chom.2013.02.007
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@article{Yamaguchi2013ARC, title={A receptor-like cytoplasmic kinase targeted by a plant pathogen effector is directly phosphorylated by the chitin receptor and mediates rice immunity.}, author={Koji Yamaguchi and Kenta Yamada and Kazuya Ishikawa and Satomi Yoshimura and Nagao Hayashi and Kouhei Uchihashi and Nobuaki Ishihama and Mitsuko Kishi-Kaboshi and Akira Takahashi and Seiji Tsuge and Hirokazu Ochiai and Yasuomi Tada and Ko Shimamoto and Hirofumi Yoshioka and Tsutomu Kawasaki}, journal={Cell host & microbe}, year={2013}, volume={13 3}, pages={347-57} }