A real-time multiplex SYBR Green I polymerase chain reaction assay for rapid screening of salmonella serotypes prevalent in the European Union.

Abstract

A two-step real-time SYBR Green I multiplex polymerase chain reaction (PCR) assay with melting curve analysis was developed for rapid detection of 19 Salmonella serotypes frequently encountered in humans, animals, and animal-associated meat products within the European Union. The first-step single-tube reaction (Multiplex PCR I), consisting of five primer… (More)
DOI: 10.1089/fpd.2010.0768

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@article{Rajtak2011ARM, title={A real-time multiplex SYBR Green I polymerase chain reaction assay for rapid screening of salmonella serotypes prevalent in the European Union.}, author={Ursula Rajtak and Nola Leonard and Declan J Bolton and S{\'e}amus Fanning}, journal={Foodborne pathogens and disease}, year={2011}, volume={8 7}, pages={769-80} }