A rat osteogenic cell line (UMR 106-01) synthesizes a highly sulfated form of bone sialoprotein.

@article{Midura1990ARO,
  title={A rat osteogenic cell line (UMR 106-01) synthesizes a highly sulfated form of bone sialoprotein.},
  author={Ronald J. Midura and David James McQuillan and K J Benham and Larry W. Fisher and Vincent C. Hascall},
  journal={The Journal of biological chemistry},
  year={1990},
  volume={265 9},
  pages={5285-91}
}
The rat osteosarcoma cell line (UMR 106-01) synthesizes and secretes relatively large amounts of a sulfated glycoprotein into its culture medium (approximately 240 ng/10(6) cells/day). This glycoprotein was purified, and amino-terminal sequence analysis identified it as bone sialoprotein (BSP). [35S]Sulfate, [3H]glucosamine, and [3H]tyrosine were used as metabolic precursors to label the BSP. Sulfate esters were found on N- and O-linked oligosaccharides and on tyrosine residues, with about half… CONTINUE READING
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