A rapidly reversible chemical dimerizer system to study lipid signaling in living cells.

@article{Feng2014ARR,
  title={A rapidly reversible chemical dimerizer system to study lipid signaling in living cells.},
  author={Suihan Feng and Vibor Laketa and Frank Stein and Anna Rutkowska and Aidan MacNamara and Sofia Depner and Ursula Klingm{\"u}ller and Julio Saez-Rodriguez and Carsten Schultz},
  journal={Angewandte Chemie},
  year={2014},
  volume={53 26},
  pages={6720-3}
}
Chemical dimerizers are powerful tools for non-invasive manipulation of enzyme activities in intact cells. Here we introduce the first rapidly reversible small-molecule-based dimerization system and demonstrate a sufficiently fast switch-off to determine kinetics of lipid metabolizing enzymes in living cells. We applied this new method to induce and stop… CONTINUE READING