A rapid test for V(D)J recombinase activity.

  title={A rapid test for V(D)J recombinase activity.},
  author={Sacha Kallenbach and Mich{\`e}le Goodhardt and François Rougeon},
  journal={Nucleic acids research},
  volume={18 22},
We describe a rapid and easy method to assay lymphoid V(D)J recombinase activity in cultured cell lines using a self-replicating recombination substrate. The substrate for recombination is a pBluescript based (Stratagene) plasmid (pBlueRec, see Figure la) in which the lacZ gene is interrupted by a DNA fragment flanked by consensus recombination signal sequences (Fig. lb). Site specific recombination will lead to deletion of the interrupting sequences restoring the correct reading frame of LacZ… CONTINUE READING