A rapid technique for isolation of viable tumor cells from solid tumors: use of the tumor cells for induction and measurement of cell-mediated cytotoxic responses.

@article{Kedar1982ART,
  title={A rapid technique for isolation of viable tumor cells from solid tumors: use of the tumor cells for induction and measurement of cell-mediated cytotoxic responses.},
  author={Eli Kedar and Barbara L. Ikejiri and Guy D. Bonnard and Ronald B. Herberman},
  journal={European journal of cancer & clinical oncology},
  year={1982},
  volume={18 10},
  pages={991-1000}
}
A rapid and simple technique for the isolation of viable tumor cells from human and mouse solid neoplasms is described. It consists of a 5 to 10-min treatment with trypsin-collagenase-DNase mixture, followed by mechanical disaggregation of the tumor tissue and subsequently by a brief centrifugation on a discontinuous Percoll gradient. With the tumors employed, this procedure usually requires less than 1 hr and results in preparations comprising greater than 80% tumor cells with viability of 80… CONTINUE READING

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