A rapid single-step centrifugation method for determination of HDL, LDL, and VLDL cholesterol, and TG, and identification of predominant LDL subclass.

@article{Sawle2002ARS,
  title={A rapid single-step centrifugation method for determination of HDL, LDL, and VLDL cholesterol, and TG, and identification of predominant LDL subclass.},
  author={Alex Sawle and Matthew K Higgins and Marcus P Olivant and Joan A. Higgins},
  journal={Journal of lipid research},
  year={2002},
  volume={43 2},
  pages={335-43}
}
Determination of the circulating levels of plasma lipoproteins HDL, LDL, and VLDL is critical in the assessment of risk of coronary heart disease. More recently it has become apparent that the LDL subclass pattern is a further important diagnostic parameter. The reference method for separation of plasma lipoproteins is ultracentrifugation. However, current methods often involve prolonged centrifugation steps and use high salt concentrations, which can modify the lipoprotein structure and must… CONTINUE READING
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