A rapid quantitative method for detecting infectious bursal disease virus using polystyrene latex microspheres.

Abstract

A monoclonal antibody (mAb) to infectious bursal disease virus (IBDV) was bound to polystyrene latex microspheres. The microspheres agglutinated with extracts of bursae and sera from chickens infected with all strains or isolates of IBDV tested. Agglutination appeared within a 10-min reaction time. The assay could detect a 10(3.7) to 10(4.5) mean embryo infective dose (EID50) of the virus in 0.01 ml and the titer of the assay was 10- to 40-times higher than that of the agar gel precipitin test.

Statistics

0200400'98'00'02'04'06'08'10'12'14'16
Citations per Year

55 Citations

Semantic Scholar estimates that this publication has 55 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Nakamura1993ARQ, title={A rapid quantitative method for detecting infectious bursal disease virus using polystyrene latex microspheres.}, author={Tadashi Nakamura and Akihiko Kato and Zhiyue Lin and Makoto Hiraga and Tetsuo Nunoya and Yoshihiro Otaki and Seiji Ueda}, journal={Journal of virological methods}, year={1993}, volume={43 1}, pages={123-9} }