A rapid quantitative assay for activated neutrophils

  title={A rapid quantitative assay for activated neutrophils},
  author={Wesley W. Bullock and Howard R. Creamer and W. Gabler},
We present here a rapid, sensitive, and convenient assay for activated human PMNs based on detecting the decreased optical density (OD) of aggregated cell suspensions. This quantitative assay uses an ELIZA machine to measure OD changes, with time, of activated cells (5 × 105 cells/well) in microtiter plates. The assay is sensitive, detecting aggregation induced by as little as 0.0001 μg/ml of LPS, or lymphokines in Con-A-activated supernatant diluted 1/500. The assay permits analysis of 400… CONTINUE READING