A rapid protocol for sexing chick embryos (Gallus g. domesticus).

@article{Clinton1994ARP,
  title={A rapid protocol for sexing chick embryos (Gallus g. domesticus).},
  author={Michael Clinton},
  journal={Animal genetics},
  year={1994},
  volume={25 5},
  pages={361-2}
}
A method for establishing the sex of chick embryos before the appearance or morphological differences between males and females has been developed. DNA was isolated from 5-7-day-old embryos by proteinase K digestion and subjected to polymerase chain reaction (PCR) amplification with W-chromosome specific primers. Sexing can be achieved within 1 day using as little as 1 ng template DNA. 

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