A rapid phytohemagglutinin induced alteration in lymphocyte potassium permeability

@article{Segel1975ARP,
  title={A rapid phytohemagglutinin induced alteration in lymphocyte potassium permeability},
  author={George B. Segel and Marsha M. Hollander and Bruce R. Gordon and Martin R. Klemperer and Marshall A. Lichtman},
  journal={Journal of Cellular Physiology},
  year={1975},
  volume={86}
}
The exposure of rat and human lymphoid cells to mitogenic concentrations of phytohemagglutinin resulted in an apparent decrease in cellular K+ without a significant change in cellular Na+ when the cells were washed with isotonic Hepes buffered choline chloride prior to cation determination. The apparent reduction in total cellular Na+ plus K+ concentration, however, was not accompanied by a change in cell volume. We inferred that the constant cell volume could occur only if the lost… 
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Lymphocyte response to phytohemagglutinin: Intracellular volume and intracellular [K+]
TLDR
The decrease in lymphocyte water volume induced by PHA was also observed for concanavalin A which stimulates lymphocyte proliferation, but not for wheat germ lectin, an agglutinating agent which is not mitogenic, suggesting volume regulation may be closely associated with the mitogenicity of these compounds.
Potasssium transport in human blood lymphocytes treated with phytohemagglutinin.
TLDR
It is confirmed that phytohemagglutinin (PHA) rapidly enhances the uptake of potassium (K+) by human blood lymphocytes, and PHA produces a marked increase in the permeability of the lymphocyte membrane to K+, and the increase in active K+ influx in PHA-treated lymphocytes may represent a homeostatic response by the membrane K+ transport system to the increased in K+ efflux.
TRANSMEMBRANE K+ TURNOVER BY PHYTOHEMAGGLUTININ (PHA) – STIMULATED HUMAN LYMPHOCYTES
Publisher Summary This chapter focuses on transmembrane K+ turnover by phytohemagglutinin (PHA)-stimulated human lymphocytes. The alteration of the plasma membrane may be critical in the occurrence
Regulation of sodium and potassium transport in phytohemagglutinin-stimulated human blood lymphocytes.
TLDR
These studies indicate that the adaptive acceleration of Na and K transport in mitogen-treated lymphocytes is mediated by a small increase in cell Na, which is capable of increasing as the cell Na rises above normal.
Human lymphocyte potassium content during the initiation of phytohemagglutinin‐induced mitogenesis
TLDR
It is shown that K+ does not change for 24 hours following PHA treatment, which may be a compensatory change for an increased exodus, explaining the failure of K+ to change following lectin treatment.
THE SPECIFICITY OF CON A – INDUCED DECREASE IN LYMPHOCYTE POTASSIUM
Publisher Summary This chapter explores the specificity of ConA-induced decrease in lymphocyte potassium. In a study described in the chapter, normal human peripheral blood lymphocytes were separated
Total and exchangeable calcium in lymphocytes: effects of PHA and A23187.
TLDR
An increase in total cell calcium is not essential for mitogenesis; however, an increase in 45Ca exchange is closely associated with the mitogenic effects of A23187 and PHA.
THE ROLE OF CATION FLUX IN TRIGGERING AND MAINTAINING THE STIMULATED STATE IN LYMPHOCYTES
Publisher Summary This chapter discusses the role of cation flux, in particular Ca ++ , K + , and Na + in lymphocyte transformation. Studies of the action of chelators and ionophores and of Ca ++
Exodus of 42K+ and 86Rb+ from rat thymic and human blood lymphocytes exposed to phytohemagglutinin
TLDR
86Rb+ efflux was increased by 27% in rat thymic lymphocytes and by 78% in human blood lymphocytes following PHA treatment, and the rate constant for efflux, Ke, was significantly increased by PHA.
Nature of lectin‐induced alteration of potassium transfer in Ehrlich ascites tumor cells
TLDR
The way in which the lectins concanavalin A and Ricinus communis agglutinin (Ricin) alter the K+ content of Ehrlich ascites tumor cells was investigated and it is suggested that a particular membrane glycoprotein may be concerned in the efflux alteration observed.
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