A rapid miniprep method for the preparation of yeast mitochondrial DNA

@article{Defontaine1991ARM,
  title={A rapid miniprep method for the preparation of yeast mitochondrial DNA},
  author={A. Defontaine and F. M. Lecocq and J. N. Hallet},
  journal={Nucleic acids research},
  year={1991},
  volume={19 1},
  pages={185}
}
Two protocols have been recently proposed to speed up the preparation of Saccharomyces cerevisiae mitochondrial DNA (mt DNA). The first (1) is a simplified procedure derived from preexisting methods using a CsCl gradient, while the other (2) is based on the isopycnic purification of the mitochondrial fraction from lysed spheroplasts. Both the above procedures require an ultracentrifugation step. We present here a rapid, simple and inexpensive miniprep method which requires no more than usual… CONTINUE READING