A rapid microplate method for the proliferation assay of fungi and the antifungal susceptibility testing using the colorimetric microbial viability assay

  title={A rapid microplate method for the proliferation assay of fungi and the antifungal susceptibility testing using the colorimetric microbial viability assay},
  author={T. Tsukatani and H. Suenaga and M. Shiga and K. Matsumoto},
  journal={Letters in Applied Microbiology},
A rapid microplate method for the proliferation assay of fungi and the antifungal susceptibility testing using the colorimetric microbial viability assay based on the reduction in a tetrazolium salt 2‐(2‐methoxy‐4‐nitrophenyl)‐3‐(4‐nitrophenyl)‐5‐(2,4‐disulfophenyl)‐2H‐tetrazolium, monosodium salt (WST‐8) with 2‐methyl‐1,4‐napthoquinone as the electron mediator was developed. The proposed method was useful to measure the proliferation of 18 kinds of moulds and seven kinds of yeasts, including… Expand
Applicability of a colorimetric method for evaluation of lactic acid bacteria with probiotic properties.
The results suggested that the WST-8-based method could be useful for the characterization of growth and tolerance of the lactic acid producing bacteria. Expand
A colorimetric assay using tetrazolium salts with an electron mediator to evaluate yeast growth in opaque dispersed systems
Background: Traditional microbiological enumeration techniques such as colony count are time consuming. Visual evaluation of turbidity growth lacks objectivity and precision. On the other hand, theExpand
Application of WST-8 based colorimetric NAD(P)H detection for quantitative dehydrogenase assays
The results suggest that the WST-8 assay is a sensitive and rapid method for determining NAD(P)H concentration and dehydrogenase enzyme activity, which can be further applied for the high-throughput screening of dehydrogenases. Expand
Antifungal activity in vitro and in vivo of a salmon protamine peptide and its derived cyclic peptide against Candida albicans
The therapeutic potential of cyclic PP as an antifungal peptide against C. albicans is shown, which resulted in a high in vivo efficacy in a murine oral candidiasis model and protected against the development of severe candidiasis. Expand
In vitro susceptibility testing for black grain eumycetoma causative agents
In vitro susceptibility assays developed for eumycetoma causative agents proved useful in determining which compounds were able to prevent hyphal growth, but a clear correlation between in vitro inhibition in terms of the half maximal inhibitory concentration or 50% minimum inhibitory concentrations and therapeutic efficacy ass tested was not found. Expand
Searching molecular determinants of sensitivity differences towards four demethylase inhibitors in Fusarium graminearum field strains.
DMI sensitivity of F. graminearum mycelium has been largely stable between 1994 and 2010, effects of snps on sensitivity towards one DMI detected in one set of strains cannot be extrapolated to other DMIs and sets of strains and no evidence for cross-resistance was obtained. Expand
Title : Antifungal activity in vitro and in vivo of a salmon protamine peptide and its derived cyclic peptide against Candida albicans Running title : Antifungal activity of protamine derived peptides
Protamine peptides (PP) derived from salmon are 14-mer consisting of 10 arginine residues. We investigated the in vitro and in vivo antifungal activity of PP against Candida albicans. PP showed theExpand


Colorimetric microbial viability assay based on reduction of water-soluble tetrazolium salts for antimicrobial susceptibility testing and screening of antimicrobial substances.
The results suggest that the WST-8 colorimetric assay is a useful method for rapid determination of the susceptibility of various bacteria to antibiotics. Expand
Colorimetric cell proliferation assay for microorganisms in microtiter plate using water-soluble tetrazolium salts.
A colorimetric method to assay cell proliferation of microorganisms in 96-well microtiter plates using water-soluble tetrazolium salts and electron mediators was developed. Combinations of 6 kinds ofExpand
Colorimetric Assay for Antifungal Susceptibility Testing ofAspergillus Species
Under these settings, the formazan production correlated linearly with the fungal biomass and less-variable concentration effect curves for amphotericin B and itraconazole were obtained. Expand
Rapid Susceptibility Testing of Medically Important Zygomycetes by XTT Assay
The use of the XTT method for rapid MIC determination for Zygomycetes is supported after significant metabolic activity was demonstrated before visual or spectrophotometric detection of fungal growth by performing the X TT assay as early as 6 h after inoculation. Expand
Comparison of a 2,3-Bis(2-Methoxy-4-Nitro-5-Sulfophenyl)-5-[(Phenylamino)Carbonyl]-2H-Tetrazolium Hydroxide (XTT) Colorimetric Method with the Standardized National Committee for Clinical Laboratory Standards Method of Testing Clinical Yeast Isolates for Susceptibility to Antifungal Agents
The data suggest that an XTT-based method could provide a useful means for the determination of antifungal susceptibility of yeasts. Expand
In Vitro Susceptibilities of Madurella mycetomatis to Itraconazole and Amphotericin B Assessed by a Modified NCCLS Method and a Viability-Based 2,3-Bis(2-Methoxy-4-Nitro-5- Sulfophenyl)-5-[(Phenylamino)Carbonyl]-2H- Tetrazolium Hydroxide (XTT) Assay
The XTT assay for fungal viability was reproducible and sensitive for both antifungals and itraconazole was superior to amphotericin B. Expand
Use of high inoculum for early metabolic signalling and rapid susceptibility testing of Aspergillus species.
This newly developed high- inoculum-based method provides rapid and reproducible MIC determinations for Aspergillus spp. Expand
In Vitro Pharmacodynamics of Amphotericin B, Itraconazole, and Voriconazole against Aspergillus, Fusarium, and Scedosporium spp
In vitro pharmacodynamics of amphotericin B, itraconazole, and voriconazole against Aspergillus, Fusarium, and Scedosporium species with a combination of two non-culture-based techniques are compared to highlight the species-specific differences in antifungal pharmacodynamics between mold-active agents that could be relevant for the development of in vitro susceptibility breakpoints and antIFungal dosing in vivo. Expand
Comparison between the Standardized Clinical and Laboratory Standards Institute M38-A2 Method and a 2,3-Bis(2-Methoxy-4-Nitro-5-[(Sulphenylamino)Carbonyl]-2H-Tetrazolium Hydroxide- Based Method for Testing Antifungal Susceptibility of Dermatophytes
The results revealed that the XTT assay can be a useful tool for antifungal susceptibility testing of dermatophytes and the agreement within 2 dilutions between these two methods was 100% for all tested drugs. Expand
Antifungal drug resistance: mechanisms, epidemiology, and consequences for treatment.
  • M. Pfaller
  • Medicine
  • The American journal of medicine
  • 2012
Standardized methods for reliable in vitro antifungal susceptibility testing are now available from the Clinical and Laboratory Standards Institute (CLSI) in the United States and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) in Europe. Expand