A rapid micro chromatin immunoprecipitation assay (ChIP)

@article{Dahl2008ARM,
  title={A rapid micro chromatin immunoprecipitation assay (ChIP)},
  author={J. A. Dahl and P. Collas},
  journal={Nature Protocols},
  year={2008},
  volume={3},
  pages={1032-1045}
}
  • J. A. Dahl, P. Collas
  • Published 2008
  • Biology, Medicine
  • Nature Protocols
  • Interactions of proteins with DNA mediate many critical nuclear functions. Chromatin immunoprecipitation (ChIP) is a robust technique for studying protein–DNA interactions. Current ChIP assays, however, either require large cell numbers, which prevent their application to rare cell samples or small-tissue biopsies, or involve lengthy procedures. We describe here a 1-day micro ChIP (μChIP) protocol suitable for up to eight parallel histone and/or transcription factor immunoprecipitations from a… CONTINUE READING

    Figures, Tables, and Topics from this paper.

    Explore Further: Topics Discussed in This Paper

    Automated microfluidic chromatin immunoprecipitation from 2,000 cells.
    • 82
    • PDF
    The Current State of Chromatin Immunoprecipitation
    • 225
    Whole-genome chromatin profiling from limited numbers of cells using nano-ChIP-seq
    • 161
    Fast genomic μChIP-chip from 1,000 cells
    • 38

    References

    Publications referenced by this paper.
    SHOWING 1-10 OF 46 REFERENCES
    Fast chromatin immunoprecipitation assay
    • 189
    • PDF
    Immunoprecipitation of native chromatin: NChIP.
    • 203