A rapid method to screen fungi for resistance to toxic chemicals


Although many species of fungi are able to degrade highly toxic chemicals, only a few species have been evaluated for resistance to toxic effects of these chemicals. In this paper we demonstrate the successful application of a method to rapidly screen several species of fungi for toxicity to chemicals or mixtures of chemicals using pentachlorophenol (PCP) as a model toxic compound. Cellulose antibiotic assay disks were soaked in solutions containing different concentrations of PCP (5, 10, 25, 50, and 80 mg l−1) and then placed in a triangular pattern outside the growing edge of the mycelia of eighteen species of white rot fungi. The plates were incubated and observed for development of inhibition zones (non-growth areas) around the disks. The short-term (24 h) growth of all eighteen species of fungi was inhibited by 5–10 mg-PCP l−1, a range similar to that observed using previously reported techniques. Long-term growth studies using this screening method were not useful since PCP diffused from the disk into the agar, decreasing the applied dose.

DOI: 10.1007/BF00702329

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@article{Alleman2004ARM, title={A rapid method to screen fungi for resistance to toxic chemicals}, author={Bruce C. Alleman and Bruce E Logan and Robert L. Gilbertson}, journal={Biodegradation}, year={2004}, volume={4}, pages={125-129} }