A rapid method to attain isotope labeled small soluble peptides for NMR studies.

Abstract

A widely applicable strategy is presented for efficient and rapid production of small water soluble peptides expressed as fusion proteins with the immunoglobulin-binding domain of streptococcal protein G. A simple extraction and purification scheme that includes a protease cleavage step to release the target peptide is described. The yield of authentic… (More)

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Cite this paper

@article{Koenig2003ARM, title={A rapid method to attain isotope labeled small soluble peptides for NMR studies.}, author={Bernd W. Koenig and Marco Rogowski and John Louis}, journal={Journal of biomolecular NMR}, year={2003}, volume={26 3}, pages={193-202} }