A rapid method of total lipid extraction and purification.

  title={A rapid method of total lipid extraction and purification.},
  author={E. Graham Bligh and W. J. Dyer},
  journal={Canadian journal of biochemistry and physiology},
  volume={37 8},
Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. [] Key Method The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non…

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