Corpus ID: 37643480

A rapid method for the mass spectrometric identification of glucuronides and other polar drug metabolites in permethylated rat bile.

  title={A rapid method for the mass spectrometric identification of glucuronides and other polar drug metabolites in permethylated rat bile.},
  author={R. Mac Thompson and Nicholas Gerber and R. A. Seibert and Dominic M. Desiderio},
  journal={Drug metabolism and disposition: the biological fate of chemicals},
  volume={1 2},
A technique for rapidly screening drugs and other compounds for possible glucuronide formation has been developed. The drug is either added to the perfusate of an isolated perfused rat liver or injected directly into the portal vein of an anesthetized rat. Bile is collected, dried, and permethylated with dimethyl sulfoxide sodium salt and methyl iodide. The product mixture is analyzed by mass spectrometry through both the direct and gas chromatography inlets. Glucuronides are identified by the… Expand
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