A rapid method for recombination and site-specific mutagenesis by placing homologous ends on DNA using polymerase chain reaction.

@article{Jones1991ARM,
  title={A rapid method for recombination and site-specific mutagenesis by placing homologous ends on DNA using polymerase chain reaction.},
  author={D. Holstead Jones and Bruce H Howard},
  journal={BioTechniques},
  year={1991},
  volume={10 1},
  pages={62-6}
}
We have developed a novel polymerase chain reaction (PCR) method that permits the rapid generation of site-specific mutants and recombinant DNA constructs with a minimum number of steps and primers. DNA segments are modified by using amplifying primers that add homologous ends to the polymerase chain reaction product(s). These homologous ends undergo recombination in vivo following transformation of recA-E. coli strains used routinely in cloning. In vivo circularization of PCR products… CONTINUE READING

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