A rapid method for isolation of human mononuclear cells free of significant platelet contamination.

@article{Casale1982ARM,
  title={A rapid method for isolation of human mononuclear cells free of significant platelet contamination.},
  author={Thomas B. Casale and Michael Kaliner},
  journal={Journal of immunological methods},
  year={1982},
  volume={55 3},
  pages={347-53}
}
In order to obtain mononuclear cells from peripheral human blood for the study of cell surface receptors, it was necessary to effectively eliminate contaminating platelets. The usual Hypaque-Ficoll isolation procedures were found to produce mononuclear cells contaminated with 10-1000 platelets per mononuclear cell (by phase microscopy). Multiple slow speed… CONTINUE READING