A rapid manual method for CD4+ T-cell quantitation for use in developing countries.


OBJECTIVE To evaluate a manual method (Cytosphere) for quantifying CD4+ T-cell numbers. DESIGN Cross-sectional study of HIV-1-seronegative and HIV-1-seropositive individuals evaluated for absolute CD4 counts by both standardized flow cytometric measurements and manual Cytosphere technology using a hemacytometer. SETTING University research hospitals in… (More)