A rapid latex agglutination assay for the detection of penicillin-binding protein 2'.

@article{Nakatomi1998ARL,
  title={A rapid latex agglutination assay for the detection of penicillin-binding protein 2'.},
  author={Yasuo Nakatomi and Jun'ichi Sugiyama},
  journal={Microbiology and immunology},
  year={1998},
  volume={42 11},
  pages={739-43}
}
A simple and rapid slide latex agglutination assay was developed to detect penicillin-binding protein 2' (PBP2') from isolates of staphylococi. PBP2' present in the membranes of methicillin-resistant Staphylococcus aureus (MRSA) or methicillin-resistant coagulase negative staphylococci (MRCNS) was rapidly extracted by alkaline treatment and, by combining with a slide agglutination reaction using latex particles sensitized with monoclonal antibodies raised against it, PBP2' could be detected… CONTINUE READING