A rapid isolation and identification method for blocked N-terminal peptides by isothiocyanate-coupled magnetic nanoparticles and MS.

@article{Zhao2009ARI,
  title={A rapid isolation and identification method for blocked N-terminal peptides by isothiocyanate-coupled magnetic nanoparticles and MS.},
  author={Li-yan Zhao and Yangjun Zhang and Junying Wei and Dong Cao and Kehui Liu and Xiaohong Qian},
  journal={Proteomics},
  year={2009},
  volume={9 18},
  pages={4416-20}
}
A quick isolation and identification of N-blocked peptides from protein digest mixtures were achieved by diisothiocyanate or isothiocyanate-coupled magnetic nanoparticles and MS. After protein digests were guanidinated and then mixed with diisothiocyanate or isothiocyanate-coupled magnetic nanoparticles, unmodified N-terminal peptides were covalently bound to magnetic nanoparticles, and can be removed from the mixture under magnetic field. Therefore, N-blocked peptides could be isolated and… CONTINUE READING