A rapid high-throughput method for mapping ribonucleoproteins (RNPs) on human pre-mRNA.

Abstract

Sequencing RNAs that co-immunoprecipitate (co-IP) with RNA binding proteins has increased our understanding of splicing by demonstrating that binding location often influences function of a splicing factor. However, as with any sampling strategy the chance of identifying an RNA bound to a splicing factor is proportional to its cellular abundance. We have… (More)
DOI: 10.3791/1622

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Cite this paper

@article{Watkins2009ARH, title={A rapid high-throughput method for mapping ribonucleoproteins (RNPs) on human pre-mRNA.}, author={Katherine H Watkins and A Stieler Stewart and William G. Fairbrother}, journal={Journal of visualized experiments : JoVE}, year={2009}, volume={34} }