Corpus ID: 34637642

A rapid fluorimetric screening method for the 1,4-benzodiazepines: determination of their metabolite oxazepam in urine.

@article{Tejedor2007ARF,
  title={A rapid fluorimetric screening method for the 1,4-benzodiazepines: determination of their metabolite oxazepam in urine.},
  author={A. M. Gil Tejedor and P. Fern{\'a}ndez Hernando and J. S. Durand Alegr{\'i}a},
  journal={Analytica chimica acta},
  year={2007},
  volume={591 1},
  pages={
          112-5
        }
}
Oxazepam is the major metabolite screened in urine samples for the evidence of the use of benzodiazepine drugs. The methods currently used, however, are laborious and time consuming. This paper proposes an oxazepam detection method based on its hydrolysis and cyclization--a reaction catalysed by cerium (IV) in an ortho-phosphoric acid-containing medium--to form 2-chloro-9(10H)-acridinone, a strongly fluorescent molecule. The variables involved in the hydrolysis and cyclization stages were… Expand