A rapid-flow perfusion chamber for high-resolution microscopy.

  title={A rapid-flow perfusion chamber for high-resolution microscopy.},
  author={Dana Kaplan and Peter Mark Bungay and J. Sullivan and Joshua Zimmerberg},
  journal={Journal of microscopy},
  volume={181 Pt 3},
Perfusion chambers employing laminar flow have dead volumes and unstirred layers which limit the minimum time required to effect a change in the local chemical environment of the sample. We have fabricated and tested a chamber capable of developing turbulent flow at reasonable flow rates of aqueous solutions. Transition to turbulence occurred at approximately equal to 1 mLs-1. To minimize dead space, a dual-exit cross-flow pattern was employed. The chamber was designed to mount on optical… CONTINUE READING