A rapid colorimetric assay with the tetrazolium salt MTT and phenazine methosulfate (PMS) for viability of Entamoeba histolytica.

@article{CedilloRivera1992ARC,
  title={A rapid colorimetric assay with the tetrazolium salt MTT and phenazine methosulfate (PMS) for viability of Entamoeba histolytica.},
  author={Roberto Cedillo-Rivera and Alejandro Alavez Ram{\'i}rez and O. Dur{\'a}n Mu{\~n}oz},
  journal={Archives of medical research},
  year={1992},
  volume={23 2},
  pages={
          59-61
        }
}
A rapid colorimetric assay for viability of E. histolytica trophozoites using the tetrazolium salt MTT and phenazine methosulfate (PMS) was developed. The MTT is normally reduced by dehydrogenases of viable cells and transformed to formazan, a colored compound; the reaction is catalyzed by PMS. Trophozoites of E. histolytica were incubated in the presence of MTT and PMS during 15, 30, 45, 60 and 75 min, at 37 degrees C. Trophozoites killed by heat or cold were used as controls. The formazan… CONTINUE READING

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