A rapid cation-exchange HPLC method for detection and quantification of pyridinium oximes in plasma and tissue.

@article{Singh2007ARC,
  title={A rapid cation-exchange HPLC method for detection and quantification of pyridinium oximes in plasma and tissue.},
  author={Harry Singh and Deborah R Moorad-Doctor and Ruthie H. Ratcliffe and Katie Wachtel and Andr{\'e}s Felipe Caro Castillo and Gregory E. Garcia},
  journal={Journal of analytical toxicology},
  year={2007},
  volume={31 2},
  pages={
          69-74
        }
}
A rapid and sensitive assay for pyridinium oximes in plasma and tissue was developed. The method was suitable for the analysis of mono- and di-pyridinium oximes and utilizes ultrafiltration followed by cation-exchange high-performance liquid chromatography with UV detection. The assay was originally developed for the measurement of the oxime MMB-4 in plasma for which the lower limit of detection was 0.0005 pg and the limit of quantitation was 0.001 to 2.5 microg. The assay required as little as… CONTINUE READING
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