A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
@article{Bradford1976ARA, title={A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.}, author={M M Bradford}, journal={Analytical biochemistry}, year={1976}, volume={72}, pages={ 248-54 } }
230,125 Citations
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Protein determination by ultra-violet absorbancy may also suffer from severe interference by other chemicals and the results must be corrected for them2.
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SUMMARY— Under optimum conditions Acid Orange–12 will bind to the proteins of meat and eggs. The dye-binding capacity is somewhat greater for these products than the previously reported values for…
Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
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The use of Coomassie Brilliant Blue G250 perchloric acid solution for staining in electrophoresis and isoelectric focusing on polyacrylamide gels.
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Orange G dye binding for determination of protein of sow's milk.
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Equations for protein in sow's milk by Orange G dye binding procedures were: per cent protein=9.184-4.526 (absorbance) at Sensitivity 1 for samples above 6.5% protein, and per centprotein=7.317-6.061 at Sensitive 2 for samples containing 3.0 to 6.0% protein.