A rapid and robust assay for detection of S-phase cell cycle progression in plant cells and tissues by using ethynyl deoxyuridine

@inproceedings{Kotogny2009ARA,
  title={A rapid and robust assay for detection of S-phase cell cycle progression in plant cells and tissues by using ethynyl deoxyuridine},
  author={Edit Kotog{\'a}ny and D{\'e}nes Dudits and G{\'a}bor V. Horv{\'a}th and Ferhan Ayaydin},
  booktitle={Plant Methods},
  year={2009}
}
Progress in plant cell cycle research is highly dependent on reliable methods for detection of cells replicating DNA. Frequency of S-phase cells (cells in DNA synthesis phase) is a basic parameter in studies on the control of cell division cycle and the developmental events of plant cells. Here we extend the microscopy and flow cytometry applications of the recently developed EdU (5-ethynyl-2'-deoxyuridine)-based S-phase assay to various plant species and tissues. We demonstrate that the… CONTINUE READING