A rapid and highly efficient method for preparation of competent Escherichia coli cells.

@article{Nishimura1990ARA,
  title={A rapid and highly efficient method for preparation of competent Escherichia coli cells.},
  author={A. Nishimura and M. Morita and Y. Nishimura and Y. Sugino},
  journal={Nucleic acids research},
  year={1990},
  volume={18 20},
  pages={
          6169
        }
}
  • A. Nishimura, M. Morita, +1 author Y. Sugino
  • Published 1990
  • Biology, Medicine
  • Nucleic acids research
  • This protocol describes a simple and efficient procedure for preparation and storage of competent Escherichia coli cells applicable to transformation and transfection. This procedure is much simpler and more reproducible than other similar methods, and allows much higher transformation efficiency (1) even after 3 months storage of the competent cells. It is also superior to the electroporation (2) in that it does not require any expensive apparatus, and gives comparable, but more consistent… CONTINUE READING
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