A rapid and efficient transformation protocol for the grass Brachypodium distachyon

@article{Christiansen2004ARA,
  title={A rapid and efficient transformation protocol for the grass Brachypodium distachyon},
  author={Pernille Christiansen and Claus H. Andersen and Thomas Didion and Marianne Folling and Klaus Kristian Nielsen},
  journal={Plant Cell Reports},
  year={2004},
  volume={23},
  pages={751-758}
}
A fast and efficient microprojectile bombardment-mediated transformation protocol is reported for the grass species Brachypodium distachyon, a proposed alternative model plant to Oryza sativa for functional genomics in grasses. Embryogenic calli derived from immature embryos were transformed by a construct containing the uidA (coding for β-glucuronidase) and bar (coding for phosphinothricin acetyl transferase) genes, and bialaphos, a non-selective herbicide, was used as the selection agent… 
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TLDR
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TLDR
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Agrobacterium-Mediated Transformation of Brachypodium distachyon.
TLDR
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Virus induced gene silencing in Brachypodium distachyon, a model organism for cereals
TLDR
This study is reporting for the first time, an implementation of virus induced gene silencing (VIGS), a powerful method allowing rapid and effective means of loss of gene function through RNA interference in this model organism.
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