A rapid and efficient transformation protocol for the grass Brachypodium distachyon

  title={A rapid and efficient transformation protocol for the grass Brachypodium distachyon},
  author={Pernille Christiansen and Claus H. Andersen and Thomas Didion and Marianne Folling and Klaus Kristian Nielsen},
  journal={Plant Cell Reports},
A fast and efficient microprojectile bombardment-mediated transformation protocol is reported for the grass species Brachypodium distachyon, a proposed alternative model plant to Oryza sativa for functional genomics in grasses. Embryogenic calli derived from immature embryos were transformed by a construct containing the uidA (coding for β-glucuronidase) and bar (coding for phosphinothricin acetyl transferase) genes, and bialaphos, a non-selective herbicide, was used as the selection agent… 
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An efficient method for transient gene expression in monocots applied to modify the Brachypodium distachyon cell wall.
This newly developed approach is a quick and efficient technique for expressing genes of interest in Brachypodium plants, which express the gene product throughout development and could be used for broad functional genomics studies of monocots and for biotechnological applications, such as plant biomass modification for biofuel production.
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Agrobacterium-mediated transformation of the temperate grass Brachypodium distachyon (genotype Bd21) for T-DNA insertional mutagenesis.
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Agrobacterium-mediated transformation of Brachypodium distachyon.
An efficient procedure enabling the Agrobacterium-mediated transformation of a range of diploid and polyploid genotypes of Brachypodium is described, which made possible the mass production of Bd21T-DNA mutant plant lines.
Agrobacterium-Mediated Transformation of Brachypodium distachyon.
A method for Agrobacterium-mediated transformation of the inbred B. distachyon lines Bd21 and Bd3-3 is described, which has been shown to be efficient and facile in several studies that resulted in the creation of over 22,000 T-DNA mutants.
Virus induced gene silencing in Brachypodium distachyon, a model organism for cereals
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Plant regeneration and micropropagation of Brachypodium distachyon
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Co-transformed, diploid Lolium perenne (perennial ryegrass), Lolium multiflorum (Italian ryegrass) and Lolium temulentum (darnel) plants produced by microprojectile bombardment
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The herbicide-tolerant zoysiagrass will permit more efficient weed control in this widely cultivated turf grass.
Agrobacterium tumefaciens-mediated transformation of Festuca arundinacea (Schreb.) and Lolium multiflorum (Lam.)
Abstract.Agrobacterium tumefaciens strain LBA4404 carrying plasmid pTOK233 encoding the hygromycin resistance (hph) and β-glucuronidase (uidA) genes has been used to transform two agronomic grass
Generation of large numbers of independently transformed fertile perennial ryegrass (Lolium perenne L.) plants of forage- and turf-type cultivars
Molecular evidence for sexual transgene transmission in fertile transgenic perennial ryegrass plants is reported for the first time, following Biolistic transfer of a ubiquitin promoter driven nptII expression cassette into mature or immature tissue derived calli followed by paromomycin selection.
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Young leaf tissue of orchardgrass was placed on Schenk and Hildebrandt medium containing 30 µM dicamba and microprojectiles coated with DNA containing the selectable bar gene (Basta® tolerance) and the reporter gene uidA coding for β-glucuronidase (GUS) were propelled into the tissue with a particle inflow gun.