A rapid affinity method for isolation and characterization of sequence specific DNA binding factor.


An affinity chromatography technique using avidin monomer and biotin interaction was used for isolating a sequence specific DNA binding factor. This approach was used for purifying a transcription factor that binds to 152 to 160 base pairs upstream of the transcription start site of adenovirus IVa2 promoter. A 432 bp IVa2 promoter fragment was isolated and… (More)


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