A rapid, non-radioactive method for the detection of insulin-like growth factor binding proteins by Western ligand blotting.

Abstract

Insulin-like growth factor binding proteins (IGFBPs) are produced by many cell types and are present in a wide variety of biological fluids. Detection of IGFBPs in complex fluids is commonly performed by Western ligand blotting using 12SI-IGF-I and/or 125I-IGF-II as the labeling ligand. This method is extremely useful in detecting IGFBPs; however, it… (More)

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