A rapid, colourimetric assay for cytotoxin activity in Campylobacter jejuni.

@article{Coote1996ARC,
  title={A rapid, colourimetric assay for cytotoxin activity in Campylobacter jejuni.},
  author={John Graham Coote and Taraq M. Arain},
  journal={FEMS immunology and medical microbiology},
  year={1996},
  volume={13 1},
  pages={65-70}
}
Cell extracts and culture supernates of Campylobacter jejuni NCTC 11168 and three isolates from faecal samples from patients with enteritis were tested for cytotoxic activity on HeLa and Vero cells using a sensitive and rapid dye reduction assay which represents a simple assay for cytotoxin activity that can be assessed visually or spectrophotometrically in the wells of microplates. The assay was as sensitive as trypan blue exclusion and did not require the use of radioisotopes. A low level of… CONTINUE READING

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