A radioisotopic assay of picomolar concentrations of coenzyme A in liver tissue.

@article{Knights1988ARA,
  title={A radioisotopic assay of picomolar concentrations of coenzyme A in liver tissue.},
  author={Kathleen M Knights and Renee Drew},
  journal={Analytical biochemistry},
  year={1988},
  volume={168 1},
  pages={94-9}
}
A single-step enzyme assay using [14C]palmitic acid and bacterial acyl-coenzyme A synthetase (EC 6.2.1.3) is described for the determination of reduced coenzyme A (CoASH) levels in liver samples. Use of this technique provides a rapid and accurate determination of CoASH in the range 1-250 pmol. Application of the method to the quantitation of CoASH in samples of human liver tissue and rat liver homogenate, isolated hepatocytes, and mitochondria is described. 

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