A radioenzymatic assay for free and conjugated normetanephrine and octopamine excretion in man.

@article{Kobayashi1980ARA,
  title={A radioenzymatic assay for free and conjugated normetanephrine and octopamine excretion in man.},
  author={Katsunobu Kobayashi and A B Foti and V Dequattro and Rainer Kolloch and Lucio Miano},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={1980},
  volume={107 3},
  pages={163-73}
}
A radioenzymatic method for the measurement of free and conjugated normetanephrine (NMN) and octopamine (OCT) is described for human urine. The assay is based on the conversion of NMN or OCT to radiolabeled metanephrine (MN) or synephrine (SYN) by phenylethanolamine-N-methyl transferase (PNMT), using tritium-labeled S-adenosyl-methionine [3H]SAM as methyl donor. Thin-layer chromatographic separation yields an assay of high specificity. The sensitivity of the assay is 5 and 2.5 pg of NMN or OCT… CONTINUE READING