A radiation hybrid transcript map of the mouse genome.


Expressed-sequence tag (EST) maps are an adjunct to sequence-based analytical methods of gene detection and localization for those species for which such data are available, and provide anchors for high-density homology and orthology mapping in species for which large-scale sequencing has yet to be done. Species for which radiation hybrid-based transcript maps have been established include human, rat, mouse, dog, cat and zebrafish. We have established a comprehensive first-generation-placement radiation hybrid map of the mouse consisting of 5,904 mapped markers (3,993 ESTs and 1,911 sequence-tagged sites (STSs)). The mapped ESTs, which often originate from small-EST clusters, are enriched for genes expressed during early mouse embryogenesis and are probably different from those localized in humans. We have confirmed by in situ hybridization that even singleton ESTs, which are usually not retained for mapping studies, may represent bona fide transcribed sequences. Our studies on mouse chromosomes 12 and 14 orthologous to human chromosome 14 show the power of our radiation hybrid map as a predictive tool for orthology mapping in humans.

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@article{Avner2001ARH, title={A radiation hybrid transcript map of the mouse genome.}, author={Philip R Avner and Thomas Bruls and Isabelle Poras and Lorraine Eley and Shahinaz Gas and Perla Ruiz and Michael V. Wiles and Rita Sousa-Nunes and Ross NW Kettleborough and Aditi Rana and Jean Morissette and Liz Bentley and Michelle E. Goldsworthy and Alyssa S Haynes and Engelhard Herbert and Lorraine Southam and Hans Lehrach and Jean Weissenbach and Giacomo Manenti and Patricia Rodriguez-Tom{\'e} and Rosa S.P Beddington and Sally L Dunwoodie and Roger D . Cox}, journal={Nature genetics}, year={2001}, volume={29 2}, pages={194-200} }