A quick method to estimate the T-DNA copy number in transgenic plants at an early stage after transformation, using inverse PCR

@article{Does1991AQM,
  title={A quick method to estimate the T-DNA copy number in transgenic plants at an early stage after transformation, using inverse PCR},
  author={Mirjam P. Does and Ben M. M. Dekker and Marcel J. de Groot and Remko Offringa},
  journal={Plant Molecular Biology},
  year={1991},
  volume={17},
  pages={151-153}
}
Agrobacterium-mediated transformation of plants is known to result in transgenic plants with a variable number of integrated T-DNA copies [1, 2, 3, 7]. Our aim was to obtain transgenic tobacco plants containing one integrated T-DNA copy per genome. Therefore, a quick method was developed to estimate the T-DNA copy number of young transgenic plantlets within 10 weeks after transformation. Inverse polymerase chain reaction (IPCR) was used to amplify junction fragments, i.e. plant genomic DNA… CONTINUE READING

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