A quick and efficient system for antibiotic-free expression of heterologous genes in tobacco roots

@article{Komarnytsky2004AQA,
  title={A quick and efficient system for antibiotic-free expression of heterologous genes in tobacco roots},
  author={Slavko Komarnytsky and Alain J. Gaume and Alison S Garvey and Nikolai Borisjuk and Ilya Raskin},
  journal={Plant Cell Reports},
  year={2004},
  volume={22},
  pages={765-773}
}
Requirement for antibiotic-resistance selection markers and difficulty in identifying transgenes with the highest expression levels remain the major obstacles for rapid production of recombinant proteins in plants. An alternative approach to producing transgenic plants free of antibiotic-resistance markers is the phenotypic-based selection with root-proliferation genes (rol genes) of Agrobacterium rhizogenes. By using Agrobacterium tumefaciens harboring the pRYG transformation vector with a… CONTINUE READING

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A revised medium for rapid growth and bioassays with tobacco tissue culture

  • T Murashige, F Skoog
  • Physiol Plant
  • 1962
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