A quantitative tool to distinguish isobaric leucine and isoleucine residues for mass spectrometry-based de novo monoclonal antibody sequencing.

Abstract

De novo sequencing by mass spectrometry (MS) allows for the determination of the complete amino acid (AA) sequence of a given protein based on the mass difference of detected ions from MS/MS fragmentation spectra. The technique relies on obtaining specific masses that can be attributed to characteristic theoretical masses of AAs. A major limitation of de… (More)
DOI: 10.1007/s13361-014-0892-1

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Cite this paper

@article{Poston2014AQT, title={A quantitative tool to distinguish isobaric leucine and isoleucine residues for mass spectrometry-based de novo monoclonal antibody sequencing.}, author={Chloe N Poston and Richard E. Higgs and Jinsam You and Valentina Gelfanova and John E. S. Hale and Michael D. Knierman and Robert W. Siegel and Jesus Aguirre Guti{\'e}rrez}, journal={Journal of the American Society for Mass Spectrometry}, year={2014}, volume={25 7}, pages={1228-36} }