A quantitative real-time PCR assay for the highly sensitive and specific detection of human faecal influence in spring water from a large alpine catchment area.

@article{Reischer2007AQR,
  title={A quantitative real-time PCR assay for the highly sensitive and specific detection of human faecal influence in spring water from a large alpine catchment area.},
  author={Georg H Reischer and David C. Kasper and Ralf Steinborn and A H Farnleitner and Robert L. Mach},
  journal={Letters in applied microbiology},
  year={2007},
  volume={44 4},
  pages={351-6}
}
AIMS The aim of the study was the development of a sensitive human-specific quantitative real-time PCR assay for microbial faecal source tracking (MST) in alpine spring water. The assay detects human-specific faecal DNA markers (BacH) from 16S rRNA gene sequences from the phylum Bacteroidetes using TaqMan minor groove binder probes. METHODS AND RESULTS The qualitative and quantitative detection limits of the PCR assay were 6 and 30 marker copies, respectively. Specificity was proved by… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 42 extracted citations

State of the art molecular markers for fecal pollution source tracking in water

Applied Microbiology and Biotechnology • 2010
View 4 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…