A quantitative polymerase chain reaction method for the detection in avian faeces of salmonellas carrying the spvR gene.

@article{Mahon1993AQP,
  title={A quantitative polymerase chain reaction method for the detection in avian faeces of salmonellas carrying the spvR gene.},
  author={J W Mahon and Alistair J. Lax},
  journal={Epidemiology and infection},
  year={1993},
  volume={111 3},
  pages={455-64}
}
A quick, semi-quantitative method of detecting Salmonella species which contain the virulence plasmid has been developed using the polymerase chain reaction (PCR). A pair of primers have been synthesized encompassing a 500 bp fragment of the spvR virulence gene. Competitor DNA consisting of the spvR gene with a 94 bp deletion situated between the primer… CONTINUE READING