A quantitative method for measuring phototoxicity of a live cell imaging microscope.

@article{Tinevez2012AQM,
  title={A quantitative method for measuring phototoxicity of a live cell imaging microscope.},
  author={Jean-Yves Tinevez and Joe M Dragavon and Lamya Baba-Aissa and P. Roux and Emmanuelle Perret and Astrid Canivet and Vincent Galy and Spencer Shorte},
  journal={Methods in enzymology},
  year={2012},
  volume={506},
  pages={291-309}
}
Fluorescence-based imaging regimes require exposure of living samples under study to high intensities of focused incident illumination. An often underestimated, overlooked, or simply ignored fact in the design of any experimental imaging protocol is that exposure of the specimen to these excitation light sources must itself always be considered a potential source of phototoxicity. This can be problematic, not just in terms of cell viability, but much more worrisome in its more subtle… CONTINUE READING
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