A quantitative immuno-electronmicroscopic study of amylase and chymotrypsinogen in peri- and tele-insular cells of the rat exocrine pancreas.

@article{Posthuma1986AQI,
  title={A quantitative immuno-electronmicroscopic study of amylase and chymotrypsinogen in peri- and tele-insular cells of the rat exocrine pancreas.},
  author={G. Posthuma and J. Slot and H. Geuze},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1986},
  volume={34},
  pages={203 - 207}
}
  • G. Posthuma, J. Slot, H. Geuze
  • Published 1986
  • Biology, Medicine
  • The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Malaisse-Lagae demonstrated in 1975 that peri-insular (PI) cells and tele-insular (TI) cells produce amylase (Am) and chymotrypsinogen (Ch) in a different ratio. These biochemical measurements are in contradiction with recent observations of Bendayan (1985), who found that the Am/Ch ratio measured with the protein A-gold technique applied to ultrathin Epon sections was the same in PI and TI cells. We have previously shown (Posthuma et al., 1984) that experimentally induced changes in Am and Ch… Expand
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  • Chemistry, Medicine
  • The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
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TLDR
The combination of immunogold labelling with cryoultramicrotomy and two replica methods, i.e. freeze‐etching and label‐fracture, are discussed, including accessibility and effect of fixatives. Expand
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TLDR
Differences in labeling intensities indicated that concentration of the zymogens starts at the boundary of the RER and cis-side of the Golgi complex, which supports the view that the GolGI cisternae are involved in protein processing in both stimulated and unstimulated cells and that Golgi cisternAE and condensing vacuoles constitute a functional unit. Expand
Disproportional immunostaining patterns of two secretory proteins in guinea pig and rat exocrine pancreatic cells. An immunoferritin and fluorescence study.
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It is hypothesize that the two proteins do not travel in-parallel towards the Golgi complex, and that, compared with Chtg, Am would lag behind in the RER cisternae. Expand
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It is demonstrated that peri-insular acinar cells contain higher amounts of secretory proteins because their organelles are larger and their zymogen granules are more numerous. Expand
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  • The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
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An inhomogeneity in the staining pattern was found; specifically, the cells located in the periinsular region of many islets showed a brighter fluorescence than acinar cells in the teleinsular tissue, adding a new parameter to describe the inhomogeneous of the exocrine pancreas. Expand
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It is demonstrated that amylase is processed through the conventional RER-Golgi-granule secretory pathway in the pancreatic acinar cells, and a concomitance has been found between some sites where protein concentration occurs. Expand
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TLDR
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The arterial supplies to the rabbit exocrine and endocrine pancreas are in parallel, with most of the flow going to the exocrine portion, however, the flow to the islets is large enough to permit significant local actions of the islet hormones on theExocrine Pancreas, in confirmation of the existence of an insuloacinar portal system. Expand
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The microcirculation of the pancreas in the anaesthetized rabbit was examined by in vivo microscopy and micro-cinephotography and the arrangement of vessels within the Pancreas was demonstrated. Expand
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