A quantitative fluorometric approach for measuring the interaction of RhoGDI with membranes and Rho GTPases.

Abstract

Tight regulation of Rho GTPase-signaling functions requires the proper localization of proteins to the membrane and cytosolic compartments, which can themselves undergo reconfiguration in response to signaling events. The importance of lipid-mediated membrane signal transduction continues to emerge as a critical event in many Rho GTPase-signaling pathways. Here we describe methods for the reconstitution of lipid-modified Rho GTPases with defined lipid vesicles and how this system can be used as a real-time assay for monitoring protein-membrane interactions.

DOI: 10.1007/978-1-61779-442-1_8

Cite this paper

@article{Johnson2012AQF, title={A quantitative fluorometric approach for measuring the interaction of RhoGDI with membranes and Rho GTPases.}, author={Jared L. Johnson and Richard A. Cerione and Jon W. Erickson}, journal={Methods in molecular biology}, year={2012}, volume={827}, pages={107-19} }