A quantitative assay for the monitoring of autophagosome accumulation in different phases of the cell cycle.

@article{Kaminskyy2011AQA,
  title={A quantitative assay for the monitoring of autophagosome accumulation in different phases of the cell cycle.},
  author={Vitaliy O. Kaminskyy and Azadeh Abdi and Boris Zhivotovsky},
  journal={Autophagy},
  year={2011},
  volume={7 1},
  pages={83-90}
}
Macroautophagy is a process accompanied by the formation of double-membrane vesicles known as autophagosomes. Although in recently published reviews various methods for the detection of autophagosomes were described, a reliable technique for the automated quantitative evaluation of autophagosome accumulation is still lacking. Here we developed a new assay which is based on the fact that the number of autophagosomes is correlated with the amount of the LC3-II protein, which is specifically… CONTINUE READING

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