A quantitative TaqMan MGB real-time polymerase chain reaction based assay for detection of the causative agent of crayfish plague Aphanomyces astaci.

@article{Vrlstad2009AQT,
  title={A quantitative TaqMan MGB real-time polymerase chain reaction based assay for detection of the causative agent of crayfish plague Aphanomyces astaci.},
  author={Trude Vr{\aa}lstad and Ann Kristin Knutsen and Torstein Tengs and Arne Holst-Jensen},
  journal={Veterinary microbiology},
  year={2009},
  volume={137 1-2},
  pages={146-55}
}
Here we present the development and first validation of a TaqMan minor groove binder (MGB) real-time polymerase chain reaction (RT-PCR) method for quantitative and highly specific detection of Aphanomyces astaci, the causative agent of crayfish plague. The assay specificity was experimentally assessed by testing against DNA representative of closely related oomycetes, and theoretically assessed by additional sequence similarity analyses comparing the primers and probe sequences to available… CONTINUE READING
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References

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Showing 1-10 of 28 references

Modelling the limit of detection in real-time quantitative PCR

  • M. Burns, H. Valdivia
  • European Food Research and Technology
  • 2008
Highly Influential
4 Excerpts

Analysis of genetic diversity in the crayfish plague fungus, Aphanomyces astaci, by random amplification of polymorphic DNA

  • T. S. Huang, L. Cerenius, K. Soderhall
  • Aquaculture
  • 1994
Highly Influential
9 Excerpts

A statistical approach for evaluation of PCR results to improve the practical limit of quantification (LOQ) of GMO analyses (SIMQUANT)

  • K. G. Berdal, C. Bøydler, T. Tengs, A. Holst-Jensen
  • European Food Research and Technology
  • 2008
Highly Influential
3 Excerpts

BioEdit: a user friendly biological sequence alignment editor and analysis program for Windows 95/98/NT

  • T. A. Hall
  • Nucleic Acids Symposium Series
  • 1999
Highly Influential
1 Excerpt

The crayfish plague fungus: history and recent advances

  • K. Söderhäll, L. Cerenius
  • Freshwater Crayfish
  • 1999
Highly Influential
5 Excerpts

Physiological adaptations in an Aphanomyces astaci strain from the warm-water crayfish Procambarus clarcii

  • J. Dieguez-Uribeondo, L. Cerenius, K. Söderhäll
  • Mycological Research
  • 1995
Highly Influential
4 Excerpts

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