A quantitative PCR protocol for detecting specific Haemoproteus lineages: molecular characterization of blood parasites in a Sedge Warbler population from southern Poland

@article{Biedrzycka2014AQP,
  title={A quantitative PCR protocol for detecting specific Haemoproteus lineages: molecular characterization of blood parasites in a Sedge Warbler population from southern Poland},
  author={Aleksandra Biedrzycka and Magdalena Migalska and Wojciech Bielański},
  journal={Journal of Ornithology},
  year={2014},
  volume={156},
  pages={201-208}
}
AbstractIn evolutionary studies, blood parasites in avian populations are commonly used as a model of host–parasite interactions. The effect of mixed infections on avian hosts has recently drawn more interest, but the effects of infection with multiple blood parasites and specific parasite lineages are poorly known. A protocol for reliable detection and quantification of lineages is essential to this type of research. Here, we present a newly developed quantitative PCR (qPCR) assay using SYBR… CONTINUE READING
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